Amphotericin-B Biolab Caution For Usage

RECONSTITUTION: AMPHOTERICIN-B should be reconstituted to a concentration of 5 mg/ml by adding 10 ml of sterile water for injection without bacteriostatic agent. The vial should be immediately shaken until the colloidal dispersion is clear. The colloidal dispersion is further diluted, usually to a concentration of 0.1 mg/ml, with 5% dextrose injection. Although the pH of commercially available 5% dextrose injection usually exceeds 4.2, the pH of each container of 5% dextrose injection should be determined and, if the pH is low, it may be adjusted. Aseptic technique must be strictly observed in the preparation of the buffer.
Reconstituted AMPHOTERICIN-B or dilutions of the drug must not be used if precipitating or foreign matter is evident.
Do not reconstitute with saline solution. The use of any diluent other than the ones recommended or the presence of the bacteriostatic agent (e.g. benzyl alcohol) in the diluent may cause precipitation.